Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
Original Papers
Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH
Fuping ZHANGQing CAOJiongjia CHENGCaihua ZHANGNa ANShuping BI
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Volume 25 (2009) Issue 8 Pages 1019-1023

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Abstract

The interaction between double-strand calf thymus gland DNA (ds-DNA) and Al(III) was studied by using differential pulse voltammetry (DPV) at a hanging mercury drop electrode (HMDE), Raman spectrometry and circular dichroism (CD) spectra. It was shown that at neutral pH ds-DNA did not produce any cathodic peak at the HMDE in the potential window from −550 to −2000 mV vs. SCE. However, in the presence of Al(III), a cathodic peak was generated at about −1660 mV, which is ascribed to a reduction of adenine and cytosine residues of single denatured DNA (sd-DNA). It was concluded that ds-DNA was completely denatured to sd-DNA by Al(III) at a neutral pH. The apparent denaturing kinetic velocity constants of ds-DNA by Al(III) were derived from linear increases of the cathodic peak currents with time. When [Al(III)]×[OH]3 ≥ 2 × 10−26, the precipitation of Al(OH)3 was observed and identified by the Raman spectrum, and inductively coupled plasma atomic emission spectrometry (ICP-AES). CD spectra showed that the B-type of structure conformations of ds-DNA and related sd-DNA did not change with the increment of Al(III) from 5.0 × 10−7 to 1.0 × 10−5 M, but the corresponding absorption strengths increased. The related physiological significances and possible applications of the observations were considered.

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© 2009 by The Japan Society for Analytical Chemistry
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