Rapid Determination of Nanotoxicity Using Luminous Bacteria

Huzhi ZHENG; Li LIU; Yihui LU; Yijuan LONG; Lingling WANG; Kam-Piu HO; Kwok-Yin WONG

doi:10.2116/analsci.26.125

Notes

Rapid Determination of Nanotoxicity Using Luminous Bacteria

Huzhi ZHENG, Li LIU, Yihui LU, Yijuan LONG, Lingling WANG, Kam-Piu HO, Kwok-Yin WONG

Author information

Huzhi ZHENG
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Li LIU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Yihui LU
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Yijuan LONG
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Lingling WANG
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University
Kam-Piu HO
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University
Kwok-Yin WONG
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University

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Supplementary material

2010 Volume 26 Issue 1 Pages 125-128

DOI https://doi.org/10.2116/analsci.26.125

Details

Abstract

A new luminescence-based toxicity test using luminous bacteria as a reporting agent has been developed to determine EC₅₀ of different nanomaterials, such as gold nanoparticles and carbon nanotubes on living organisms. The whole assay takes only about 15 min and is as sensitive as other standard methods. Due to its technical simplicity, rapidity and sensitivity, this luminescent bacteria test has the potential to be developed as a general test of toxicity for a wide variety of nanomaterials.

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