2010 Volume 26 Issue 12 Pages 1265-1270
A novel label-free electrochemical nucleic acid aptasensor for the determination of cocaine by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-modified electrode is presented. When cocaine was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed, resulting in a variation of the corresponding peak current of an electrochemical probe ([Fe(CN)6]3−/4−). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to characterize modifications of the electrode surface. The peak current was detected by differential pulse voltammetry (DPV). Under the optimized experimental conditions, the presented sensor exhibits a nice specificity towards cocaine. The decrease of the peak current response of the aptasensor has a linear relationship with the concentration of cocaine ranging from 1.0 × 10−6 to 1.5 × 10−4 mol L−1 with a detection limit of 3 × 10−7 mol L−1 at 3σ. The proposed aptasensor can be easily regenerated by the denaturalization of aptamer-target complexes in a heated water bath at 80 – 90°C. Besides, this biosensor has a high reproducibility and selectivity, which can be a promising method to detect cocaine in real samples.