Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Application of an Oxygen Electrode to Evaluate Superoxide Anion-scavenging Ability
Keiko KOMAGOEHiroaki TAKEUCHITsuyoshi INOUETakashi KATSU
Author information
JOURNALS FREE ACCESS

2010 Volume 26 Issue 8 Pages 903-906

Details
Abstract

The ability to scavenge superoxide anion radicals (O2) was determined using an oxygen electrode. The method is based on the determination of O2 generated by the reaction of nitrilotriacetatoiron(III) with hydrogen peroxide and a decrease in the concentration of O2 by a scavenging reaction, converting into a change in the generation of oxygen molecules through an electron-transfer reaction from O2 to nitrilotriacetatoiron(III). Oxygen generation, which enhanced proportionally with an increase in the concentration of hydrogen peroxide, was inhibited depending on the concentration of superoxide dismutase. Hence, we applied the present reaction system to evaluate the O2-scavenging abilities of an antioxidant, measuring the degree of inhibition of oxygen generation using an oxygen electrode. A good correlation was obtained between the present method and conventional colorimetry, monitoring the formation of blueformazan by the reaction of nitro blue tetrazolium with O2, to estimate the O2-scavenging activities of antioxidants.

Information related to the author
© 2010 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top