Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Enhancement of Vindoline Production in Suspension Culture of the Catharanthus roseus Cell Line C20hi by Light and Methyl Jasmonate Elicitation
Lihong HELi YANGRonghui TANShujuan ZHAOZhibi HU
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2011 Volume 27 Issue 12 Pages 1243

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Abstract

The effects of light and methyl jasmonate (MJ) on the transcription of biosynthetic genes as well as the accumulation of vindoline and catharanthine in Catharanthus roseus C20hi cell suspensions were studied. t16h (the gene encoding tabersonine 16-hydroxylase) could be induced by light and MJ, whereas d4h (the gene encoding deacetoxyvindoline 4-hydroxylase) could only be induced by light. Quantification by UPLC-MS showed that light significantly increased vindoline production in C20hi cells by about 0.49 – 5.51-fold more than that in controls, with the highest yield being 75.3 ng/g of dry weight. The biosynthesis of vindoline was further enhanced by combining MJ with light. The accumulation of catharanthine was not improved by either light or MJ elicitation. These results suggested that light and MJ could promote vindoline, but not catharanthine accumulation in C20hi cells.

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© 2011 by The Japan Society for Analytical Chemistry
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