Rapid and Sensitive Determination of Benzaldehyde Arising from Benzyl Alcohol Used as Preservative in an Injectable Formulation Solution Using Dispersive Liquid–Liquid Microextraction Followed by Gas Chromatography

Abstract

A rapid and sensitive method has been developed for the determination of benzaldehyde, a toxic oxidation product of the widely used preservative and co-solvent benzyl alcohol in injectable formulations of non-steroidal anti-inflammatory drugs, diclofenac, vitamin B-complex and Voltaren injection solutions by using dispersive liquid–liquid microextraction followed by gas chromatography. This method involves the use of an appropriate mixture of extraction solvent (43.0 μL 1,2-dichloroethane) and disperser solvent (1.0 mL acetonitrile) for the formation of a cloudy solution in a 5.0-mL aqueous sample containing benzaldehyde. The linear range was 1.0 – 1000 μg L⁻¹, and the limit of detection was 0.2 μg L⁻¹ for benzaldehyde.