Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Visual and On-site Detection of Mercury(II) Ions on Lateral Flow Strips Using DNA-functionalized Gold Nanoparticles
Fei YANGJing DUANMin LIZebo WANGZhiyong GUO
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JOURNAL FREE ACCESS

2012 Volume 28 Issue 4 Pages 333

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Abstract
A test strip for detection of Hg2+ in aqueous solution based on the DNA-functionalized gold nanoparticles (DNA-AuNPs) was developed and evaluated. When Hg2+ ions were introduced, the biotinylated DNA2 hybridized with thiolated DNA1 functionalized on the AuNPs (DNA1-AuNPs) to form mismatch complexes through thymine-Hg2+-thymine (T-Hg2+-T) coordination. The formed mismatch complexes and excess DNA1-AuNPs could be captured on the test line formed by streptavidin and the control line formed by DNA3-BSA, respectively. Two red lines appeared due to the accumulation of AuNPs, enabling visual detection of Hg2+ with a detection limit of about 6 nM. The assay results can be obtained within 5 min. The results show that the test strip has excellent sensitivity and selectivity for detection of Hg2+; thus it holds a great potential for rapid, on-site and real time detection of Hg2+.
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© 2012 by The Japan Society for Analytical Chemistry
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