Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Effect of a Low-conductivity Zone on Field-amplified Sample Stacking in Microchip Micellar Electrokinetic Chromatography
Kenji SUEYOSHIFumihiko KITAGAWAKoji OTSUKA
Author information
JOURNALS FREE ACCESS

2013 Volume 29 Issue 1 Pages 133-138

Details
Abstract

The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume injection of a sample solution were demonstrated. As a typical result, the observed peak intensities of the fluorescent dyes were 1.5 – 2.0-fold enhanced by partial injection of the low-conductivity zone, as compared to those in FASS-MCMEKC without injecting the low-C zone; also, 30 – 40-fold enhancements of the detectability were achieved as compared to the conventional MCMEKC analyses. The resolutions of the hydrophobic analytes were also improved by introducing the low-C zone. The difference in the retention factors of model analytes in the high-C background and low-C zones was also estimated in order to clarify the effect of the low-C zone on the resolution and detectability in FASS-MCMEKC.

Information related to the author
© 2013 by The Japan Society for Analytical Chemistry
Previous article Next article
feedback
Top