2013 Volume 29 Issue 1 Pages 21-24
A chemiluminescence method for the determination of glucose (GC) using a batch system made in our laboratory is proposed. The method is based on measures of the chemiluminesence intensity from a batch of sample GC solutions following the injection of an (NH4)2[Ce(NO3)6]–[Ru(bpy)3]Cl2 solution. The effect of the Ru complex concentration on chemiluminescence was studied. The detection limit was 0.5 × 10−8 M, and the linear calibration curve reached 10−7 M. The Ru complex was selected on the basis of its desirable performance compared to those of other photosensitizers. However, flow-injection analysis (FIA) using the Ce-Ru complex reagent was not applicable for the detection of GC.