Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method

Toyohiro NAITO; Ai YATSUHASHI; Noritada KAJI; Taeko ANDO; Kazuo SATO; Hisao MORIYA; Hiroaki KITANO; Takao YASUI; Manabu TOKESHI; Yoshinobu BABA

doi:10.2116/analsci.29.367

Notes

Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method

Toyohiro NAITO, Ai YATSUHASHI, Noritada KAJI, Taeko ANDO, Kazuo SATO, Hisao MORIYA, Hiroaki KITANO, Takao YASUI, Manabu TOKESHI, Yoshinobu BABA

Author information

Toyohiro NAITO
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
FIRST Research Center for Innovative Nanobiodevices, Nagoya University
Ai YATSUHASHI
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Noritada KAJI
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
FIRST Research Center for Innovative Nanobiodevices, Nagoya University
Taeko ANDO
College of Science and Engineering Department of Micro System Technology, Ritsumeikan University
Kazuo SATO
Department of Mechanical Engineering, Faculty of Engineering, Aichi Institute of Technology
Hisao MORIYA
Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Hiroaki KITANO
The Systems Biology Institute
Takao YASUI
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
FIRST Research Center for Innovative Nanobiodevices, Nagoya University
Manabu TOKESHI
FIRST Research Center for Innovative Nanobiodevices, Nagoya University
Division of Biotechnology and Macromolecular Chemistry, Faculty of Engineering, Hokkaido University
Yoshinobu BABA
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
FIRST Research Center for Innovative Nanobiodevices, Nagoya University
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

JOURNAL FREE ACCESS

2013 Volume 29 Issue 3 Pages 367-371

DOI https://doi.org/10.2116/analsci.29.367

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Abstract

A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.

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