Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Notes
Patterned Co-culture of Live Cells on a Microchip by Photocrosslinking with Benzophenone
Kiichi SATOSayaka KIKUCHIEri YOSHIDAReina ISHIINaoki SASAKIKin-ichi TSUNODAKae SATO
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2016 Volume 32 Issue 1 Pages 113-116

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Abstract

The patterned coculture of different types of living cells in a microfluidic device is crucial for the analysis of cellular interactions and cell-cell communication. In the present study, cell patterning was achieved by photocrosslinking benzophenone derivatives in a microfluidic channel. Optimization of UV irradiation conditions enabled successful fixation of live cells. In addition, patterning and co-culture of non-adherent K562 cells and adherent RF-6A cells was achieved by successive rounds of patterning. The present approach is expected to be useful for the development of in vitro methods for studying cell signaling.

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© 2016 by The Japan Society for Analytical Chemistry
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