Oxime Based Selective Fluorescent Sensor for Arsenate Ion in a Greener Way with Bio-Imaging Application

Abstract

A newly designed oxime based probe, 2,4-dihydroxyacetophenone-oxime (DHAO), was found to recognize H₂AsO₄^– selectively with ∼3 fold "turn-on" fluorescence enhancement and LOD of 29 μM, K = (2.10 ± 0.54) × 10⁴ M^–1 in purely aqueous medium. The structures of the DHAO and its adduct with H₂AsO₄^– were optimized by DFT calculations. Intracellular imaging of As(V) in HepG2 cells demonstrate the possibilities of in vitro/in vivo applications for selective monitoring of such species.