Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
A Simple Method for Ion Channel Recordings Using Fine Gold Electrode
Daichi OKUNOMinako HIRANOHiroaki YOKOTAYukiko ONISHIJunya ICHINOSEToru IDE
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2016 Volume 32 Issue 12 Pages 1353-1357

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Abstract

The artificial bilayer single-channel recording technique is commonly used to observe detailed pharmacological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we develop a novel artificial bilayer single-channel recording technique in which bilayers are made and channels are reconstituted into the membranes by contacting a gold electrode to the lipid-solution interface. Using this technique, we measured the single-channel currents of two channel-forming peptides, gramicidin and alamethicin, and a channel-forming protein, α-hemolysin. This technique requires only one action, allowing the technique to potentially be combined with high-throughput screening devices.

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© 2016 by The Japan Society for Analytical Chemistry
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