Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
Rapid Communications
Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy
Ahmed ALIYasmine ABOULEILASara AMERRie FURUSHIMASamy EMARASebastien EQUISYann COTTETsutomu MASUJIMA
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2016 Volume 32 Issue 2 Pages 125-127

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Abstract

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells’ before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.

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© 2016 by The Japan Society for Analytical Chemistry
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