BODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells

Akira TSUCHIYA; Siti N. HASHIM; Shoko ISE; Takafumi FURUHATA; Kiyohiko KAWAI; Rie WAKABAYASHI; Masahiro GOTO; Noriho KAMIYA; Shinsuke SANDO

doi:10.2116/analsci.32.543

Original Papers

BODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells

Akira TSUCHIYA, Siti N. HASHIM, Shoko ISE, Takafumi FURUHATA, Kiyohiko KAWAI, Rie WAKABAYASHI, Masahiro GOTO, Noriho KAMIYA, Shinsuke SANDO

Author information

Akira TSUCHIYA
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Siti N. HASHIM
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Shoko ISE
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Takafumi FURUHATA
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Kiyohiko KAWAI
The Institute of Scientific and Industrial Research, Osaka University
Rie WAKABAYASHI
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Masahiro GOTO
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Center for Future Chemistry, Kyushu University
Noriho KAMIYA
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
Center for Future Chemistry, Kyushu University
Shinsuke SANDO
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

Keywords: Aptamer, fluorescent sensor, IFN-γ, ATP

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Supplementary material

2016 Volume 32 Issue 5 Pages 543-547

DOI https://doi.org/10.2116/analsci.32.543

Details

Abstract

An on-cell aptamer sensor has the potential to reveal cell–cell communications by signalling molecules. We attempted to design new fluorescent aptamer sensors for the sensing of IFN-γ and adenine compounds on cells. BODIPY-labeled external quencher-free aptamer sensors have allowed a turn-on detection of the target molecule with improved off/on efficiency.

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