Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions

Tao YANG; Fang ZHU; Tianxiao ZHOU; Jianzhong CAO; Yibo XIE; Meiyi ZHANG; Yan WANG; Dong-Sheng CAO; Qinlu LIN; Lin ZHANG

doi:10.2116/analsci.33.191

Original Papers

Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions

Tao YANG, Fang ZHU, Tianxiao ZHOU, Jianzhong CAO, Yibo XIE, Meiyi ZHANG, Yan WANG, Dong-Sheng CAO, Qinlu LIN, Lin ZHANG

Author information

Tao YANG
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Fang ZHU
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Tianxiao ZHOU
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Jianzhong CAO
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Yibo XIE
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Meiyi ZHANG
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Yan WANG
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Dong-Sheng CAO
School of Pharmaceutical Sciences, Central South University
Qinlu LIN
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology
Lin ZHANG
National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology
Department of Food Science and Technology, Central South University of Forestry and Technology

Keywords: Copper ion, determination, fluorescent peptide probe, selectivity, water sample

JOURNAL FREE ACCESS

2017 Volume 33 Issue 2 Pages 191-196

DOI https://doi.org/10.2116/analsci.33.191

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Abstract

This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu²⁺) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu²⁺ via quenching of the probe fluorescence upon a binding of Cu²⁺. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu²⁺ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu²⁺ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu²⁺ in aqueous solutions.

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