Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Original Papers
Fluorescence Quenching of the Probes L-Tryptophan and Indole by Anions in Aqueous System
Muhammad IDREESMuhammad AYAZRafida BIBIMuhammad N. KHAN
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2020 Volume 36 Issue 2 Pages 183-185


A fluorescence quenching study of the fluorescent probes L-tryptophan and indole by anions (NO3, Cl, SO42−) was carried out in an aqueous system. The ions NO3, Cl and SO42− showed very good quenching of both the probes. Quenching of L-tryptophan by all the anions studied was higher as compare to the quenching of indole. The data was fitted with the Stern–Volmer equation. Stern–Volmer constants were observed in the order NO3 > Cl > SO42−. Stern–Volmer constants reflect the sensitivity of the method for the studied anions. Limit of detection (LOD) was calculated as three times the standard deviation of the blank for n = 10 (3 × SD) while the limit of quantification (LOQ) was calculated as ten times the standard deviation of the blank for n = 10 (10 × SD). In the case of L-tryptophan LOD and LOQ varied from 4.08 × 10−5 – 4.56 × 10−4 mol L−1, while in the case of indole the values ranged from 3.87 × 10−5 – 6.59 × 10−4 respectively. Fluorescence quenching of L-tryptophan and indole by the studied anions showed good reproducibility and the method could be very effective for the determination of anions.

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© 2020 by The Japan Society for Analytical Chemistry
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