High Performance Liquid Chromatography of Phosphomolybdate and Silicomolybdate: Simultaneous Determination of Phosphate and Silicate

Abstract

A method for the simultaneous determination of phosphate and silicate was established by high performance liquid chromatography as their molybdocomplexes. The excellent separation of them was performed on a column packed with Styragel 60 A (Waters Associates, lnc.) using an aqueous 55%(v/v) acetonitrile solution containing 0.75M sulfuric acid and 0.02M tetrabutylammonium hydrogensulfate as an eluent. The calibration curves for phosphate and silicate were linear in the range 0.03-10μgP/ml and 0.01-5.0μgSi/ml, respectively. The method was applied to the determination of phosphate and silicate in human urine and plasma.