Abstract
An electrochemical method of analysis, which can avoid the interference of dissolved oxygen by using twin electrodes in a flow cell, was extensively applied to a variety of quinones and carbonyls. The quantitation by this method was occasionally affected by parallel or following chemical reactions coupled with the electrolytic reduction at the first electrode (generating electrode). The loss of reductant was caused by the reoxidation by dissolved oxygen, hydrolysis and/or adsorption of reduced entity before arriving at the second electrode (detecting electrode), and sometimes the photochemical reduction interfered with the analysis. The variation of the detection limit; mitomycin C 5nmol, daunomycin 2nmol, vitamin K3 0.1nmol, actinomycin D 0.2nmol and tetracycline 0.05 nmol, was interpreted in terms of these coupled chemical reactions.
