Abstract
A fluorometric method based on the measurement of two nucleobase pairs of guanine-cytosine and adenine-thymine was developed for the accurate quantification of double-stranded DNA. Two fluorescence reactions with phenylglyoxal and chloroacetaldehyde are utilized for detection of the guanine and adenine moieties in DNA, respectively. The optimized conditions of the respective reactions permit quantitative determination of DNA at concentrations as low as 0.15μg ml-1. The DNA-content values determined for five commercial DNA preparations were 80-103% of their actual weights.
