Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Stability of an Agar-Supported Bilayer Lipid Membrane and Its Application to a Chemical Sensor
Masayuki UTOMakoto ARAKITakashi TANIGUHISuwaru HOSHISadanobu INOUE
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1994 Volume 10 Issue 6 Pages 943-946

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Abstract

Lipid membranes made of lecithin and cholesterol were formed by self-assembly in a small aperture on an agar support. The membranes exhibited an average electric resistance of 135GΩ and a capacitance of 0.43μF/cm2. Gramicidin, known to form a channel in uni-lamellar lipid bilayers, reduced the electric resistance to a MΩ level, thus showing the membranes to be of a uni-lamellar bilayer type. The membrane stability was investigated against perturbation with electric potentials and against mechanical agitation in the contacted aqueous solution. About 80% of the membrane preparations remained intact after applying electric potentials of between +1500mV and-1500mV. A similar percentage of the membranes stayed intact under 100rpm magnet stirring in a 30ml vessel. Membranes containing valinomycin responded to K+ ions with changes in both the membrane conductance and the membrane potential.

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