Optimization of the Reaction Conditions for the Peroxyoxalate Chemiluminescence Detection System of Fluorescent Compounds in a High-Performance Liquid Chromatography

Abstract

Using an instrument of automatic fluid injection (batch method), we optimized such reaction conditions as pH, concentration and kinds of buffer for the peroxyoxalate chemiluminscence (PO-CL) detection of fluorescent compounds for high-performance liquid chromatography (HPLC). The results were compared with those obtained by HPLC. Consequently, the usefulness of the batch method for investigating the reaction conditions for PO-CL detection in HPLC was demonstrated. With the optimized condition using a phosphate buffer for the eluent in HPLC, the detection limits for Dns-Val and dipyridamole were 70amol and 20amol on column (S/N=2), respectively.