Abstract
The fluorescence properties of 7-anilino-4-methylcoumarin-3-(p)-benzoic acid (AMCBA) synthesized as a fluorescent probe were measured in various solvents and in the egg albumin (EA) solution. The fluorescence of AMCBA was significantly enhanced in the nonpolar solvents and in the egg albumin solution. The binding parameters of AMCBA to EA were estimated fluorometrically. AMCBA which bound to EA was competitively displaced by several β-blockers and sulfonamide drugs. The binding parameters of β-blockers and sulfonamides to EA were estimated by using a displacement method. A good correlation between binding constants of sulfonamides or β-blockers to EA and those partition coefficients was observed. These data suggest that AMCBA is one of the useful fluorescent probes for studying the binding of drugs to polymeric pharmaceutical additives, including EA.
