Abstract
A Laser-induced confocal microscope system with the CCD-camera detection suitable both for spectroscopic investigation and for direct imaging is described. In combination with a spatial pinhole, depth discrimination was obtained. A method based on the reflected light from a quartz plate was proposed to detect the capability and a vertical resolution of 2.7μm in terms of the full width at half maximum was achieved. The spatially- registered and temporal fluorescence spectra can be acquired by this system. Without using monochromator, direct real time fluorescence imaging with temporal time as short as 0.5ms can be obtained. The potential applications of the confocal microscopy to air-liquid interface studies are discussed.
