Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
CCD-Based Confocal Laser-Microscope System For Spectroscopic Investigation And Direct Imaging
Yao Qun LIShin-ya SASAKITakanori INOUETeiichiro OGAWA
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1997 Volume 13 Issue Supplement Pages 81-84

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Abstract

A Laser-induced confocal microscope system with the CCD-camera detection suitable both for spectroscopic investigation and for direct imaging is described. In combination with a spatial pinhole, depth discrimination was obtained. A method based on the reflected light from a quartz plate was proposed to detect the capability and a vertical resolution of 2.7μm in terms of the full width at half maximum was achieved. The spatially- registered and temporal fluorescence spectra can be acquired by this system. Without using monochromator, direct real time fluorescence imaging with temporal time as short as 0.5ms can be obtained. The potential applications of the confocal microscopy to air-liquid interface studies are discussed.

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© The Japan Society for Analytical Chemistry
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