Determination of Pheophorbide a and Methyipheophorbide a by Fluorescence High Performance Liquid Chromatography: Availability of Narrow Baseline Method

Yoshiaki YOSHITAKE; Kikuko KAKIUCHI; Kiyotoshi MORISHIGE; Shiro GOHDA; Yasuharu NISHIKAWA; Tsunenobu SHIGEMATSU

doi:10.2116/analsci.2.67

Yoshiaki YOSHITAKE, Kikuko KAKIUCHI, Kiyotoshi MORISHIGE, Shiro GOHDA, Yasuharu NISHIKAWA, Tsunenobu SHIGEMATSU

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Keywords: Pheophorbide a, methylpheophorbide a, chlorophyll, fluorescence high performance liquid chromatography, narrow baseline method

JOURNAL FREE ACCESS

1986 Volume 2 Issue 1 Pages 67-69

DOI https://doi.org/10.2116/analsci.2.67

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Abstract

A narrow baseline method for the simultaneous determination of pheophorbide a and methylpheophorbide a in core sediment samples has been developed by high performance liquid chromatography. A linear relationship between the concentration of methylpheophorbide a and the peak height on the narrow baseline was obtained. The relative standard deviation of measurement was found to be 2.4% (n=6) for samples containing 8.1×10^-8mol dm^-3 ofmethylpheophorbide a.

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