Abstract
A photoacoustic cell which can accommodate a thin-layer plate of 26×76mm is described. A high-power light emitting diode was used as a visible light source to scan the plate surface to obtain a densitogram of each material. Protein dissolved in a 2M NaCl solution was spotted onto the thin-layer plate; staining with amidoblack 10B followed. The protein (e.g. bovine serum albumin, hemoglobin, myoglobin) spotted onto slica-gel thin-layer plate was determined using the apparatus. The detection limit of bovine serum albumin stained with amidoblack 10B was about 25ng, with a signal/noise ratio of 2. A densitogram of rat serum protein seperated with polyacrylamide gel electrophoresis was obtained by use of the apparatus.
