Abstract
The light scanning photoacoustic densitometry was applied to sodium dodecylsulfate polyacrylamide gel electrophoresis. A near-infrared laser diode and a light emitting diode were used as light sources. Electrophoresed protein was silver stained, followed by vacuum drying. The photoacoustic signal amplitude from 0.10μg ovalbumin depended on the chopping frequency, f, as a function of f-3/2 over 60Hz, and as f-1 below 60Hz. The background signal was reduced by increasing the chopping frequency. The detection limits of bovine serum albumin, ovalbumin, tripsiongen, and hemoglobin were about 0.6, 0.5, 0.8 and 0.3ng respectively, using a laser diode.
