Abstract
A water-soluble 9, 10-phenanthrenequinone-3-sulfonate was synthesized as a fluorogenic reagent which reacted with guanidino group in an alkaline solution. The reagent was used for the determination of guanidino compounds by high performance liquid chromatography where the mobile phase contained the fluorogenic reagent. Several guanidino compounds were separated within 20min on a Nucleosil C8 column (3μm, 75mm×4.6mm i.d.) by an isocratic, reversed phase and ion-pair chromatography and detected as fluorophores derived from condensation with the reagent in an alkaline solution. The chromatographic system, in which the mobile phase contained the reagent, was simple because only two pumps were required to deliver the mobile phase and the alkaline solution. The relative standard deviation (within-run, n=10) and the limit of detection for the assay of the compounds were 3.00% and 0.1nmol, respectively. This method was applied to the determination of guanidino compounds in serum obtained from patients on haemodialysis therapy.
