Abstract
A simple and selective method for the determination of thiocyano group was established. The principle of this method is as follows: Thiocyano group is reduced by dithiothreitol to thiol and cyanide; the latter is converted to hydrogen cyanide by acidification, and then it is absorbed to alkali solution by microdiffusion method. Then cyanide is determined according to our HPLC method with fluorometric detection based on König reaction. The calibration curve for the thiocyano group was linear over the range of 0.05 to 50μM. The present method permitted the determination of thiocyano group in bovine serum albumin and rabbit plasma treated with cyanide.
