Adsorption Effects on Retention Behavior of Hydrocarbon Solutes in Gas Chromatography by Use of Nonpolar Stationary Liquid Phase Squalane

Abstract

The solute retention mechanism in gas-liquid chromatography was studied for the hydrocarbon solute-nonpolar stationary liquid phase (squalane) system. The retention volume of the solute and the specific surface area of the liquid-coated, modified alumina were determined as a function of the liquid loading. On the basis of the previous reasoning, distribution constants for the bulk solution partition and some adsorption equilibria taking part in the solute retention could be estimated. The results prove to be quite different from those of the polar liquid phases used previously: that is, squalane formed a bulk liquid layer on the modified alumina after the solid support was completely covered with the monolayer.