Microdetermination of Unsaturated Disaccharides Produced from Chondroitin Sulfates in Rabbit Plasma by High Performance Liquid Chromatography with Fluorometric Detection

Hidenao TOYODA; Kazufusa SHINOMIYA; Shigeyuki YAMANASHI; Ichiro KOSHIISHI; Toshio IMANARI

doi:10.2116/analsci.4.381

Hidenao TOYODA, Kazufusa SHINOMIYA, Shigeyuki YAMANASHI, Ichiro KOSHIISHI, Toshio IMANARI

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Keywords: Unsaturated disaccharide, chondroitin sulfate, glycosaminoglycan, high performance liquid chromatography, 2-cyanoacetamide

JOURNAL FREE ACCESS

1988 Volume 4 Issue 4 Pages 381-384

DOI https://doi.org/10.2116/analsci.4.381

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Abstract

A highly sensitive high performance liquid chromatographic method with fluorometric postcolumn labeling using 2-cyanoacetamide was developed for determination of unsaturated disaccharides produced enzymatically from chondroitin sulfates (ChS). The calibration curves of the unsaturated disaccharides were linear over the range from 2pmol to 5nmol. This method was applied to the analysis of normal rabbit plasma for ChS to verify the sensitivity and practicality.

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