Detection of Chemiluminescent Labeling Compounds by Photographic Method. Preliminary Investigation on 1, 10-Phenanthroline-Hydrogen Peroxide and Luminol-Hydrogen Peroxide Systems

Abstract

The following two chemiluminescent reaction systems were investigated for the photographic detection of ultra-micro amounts of analyte on an agarose gel plate. 1) Reaction of 1, 10-phenanthroline with hydrogen peroxide, which is catalyzed by copper(II) or osmium(VIII). 2) Reaction of luminol with hydrogen peroxide, which is catalyzed by iron(III) complex. Chemiluminescent light was detected using a high-speed instant photographic film, and amounts as small as 9×10^-10mol of 1, 10-phenanthroline or 2×10^-18mol of iron complex of tetraphenylporphinetrisulfonic acid were detected by this method.