Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
Enzyme Electrode for Determination of Glycerophosphate by Combined Use of Glycerophosphate Oxidase and Peroxidase
Junji KOJIMANaofumi MORITAMasanosuke TAKAGI
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1988 Volume 4 Issue 5 Pages 497-500

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Abstract

An enzyme sensor for L-α-glycerophosphate was prepared by covalent fixation of glycerophosphate oxidase and peroxidase by use of bovine serum albumin and glutaraldehyde onto a glassy carbon electrode silanized with 3-aminopropyl triethoxysilane. The sensor showed good linear current responses in the range of 5 to 100μM glycerophosphate (γ=0.999, n=7); the response time was about 20s. The sensor was stable for at least 60d. Sugar phosphates did not have any appreciable effect on the response of the sensor, but reducing materials such as L-ascorbic acid and L-cysteine gave some errors that changed with concentrations.

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© The Japan Society for Analytical Chemistry
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