Simultaneous Assay of Aspartate Aminotransferase and Alanine Aminotransferase by Flow Injection Method Using Immobilized Enzyme Reactors

Abstract

A simultaneous assay of two enzymes by a flow injection analytical (FIA) method using enzyme-immobilized open- tubular reactors in parallel is described. Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were studied. This FIA system has an additional channel for evaluation and correction for the error resulting from pyruvate coexisting in sample sera. The results obtained by the FIA method corresponded well with those obtained by a conventional UV method. The recognition of the signal pattern from three channels of the FIA system will make possible the primary screening of diseases.