Fluorometric Determination of Uric Acid with 4, 5-Dimethyl-o-phenylenediamine

Abstract

A new sensitive fluorometric method for the determination of uric acid is presented. Uric acid reacted with 4, 5-dimethyl-o-phenylenediamine (DMPD) after the oxidation using N-bromosuccinimide (NBS) to give a green fluorescence with excitation maxima at 350nm and 400nm, and an emission maximum at 500nm. By the use of the fluorescence reaction, uric acid in aqueous solution was determined in the range of 0.01-5.0μg ml^-1, and the relative standard deviation for 1.0μg ml^-1 uric acid was 0.51%(n=10). The proposed method could be applied to the determination of uric acid in serum.