Determination of Serum Glucose by Isotope Dilution Mass Spectrometry after Off-Line Liquid Chromatographic Separation

Akiko TAKATSU; Sueo NISHI

doi:10.2116/analsci.5.13

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Keywords: Isotope dilution mass spectrometry, high performance liquid chromatography, serum glucose, electron impact ionization technique, chemical ionization technique

JOURNAL FREE ACCESS

1989 Volume 5 Issue 1 Pages 13-17

DOI https://doi.org/10.2116/analsci.5.13

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Abstract

Determination of serum glucose by isotope dilution mass spectrometry with liquid chromatography is described. After adding uniformly labeled glucose to the serum, serum protein was removed. Glucose was separated using high performance liquid chromatography and measured by electron impact or chemical ionization mass spectrometry with a direct insertion device. Analytical results of NBS SRM serum showed satisfactory agreement with the certified value; relative standard deviations of about 1% were obtained.

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