Simultaneous Determination of Befunolol and Its Major Metabolite, Dihydrobefunolol, in Plasma by High Performance Liquid Chromatography

Yoshihide NOZAKI; Teruko IMAI; Keiko GOTO; Yorishige IMAMURA; Masaki OTAGIRI; Willy J.M. UNDERBERG

doi:10.2116/analsci.5.395

Yoshihide NOZAKI, Teruko IMAI, Keiko GOTO, Yorishige IMAMURA, Masaki OTAGIRI, Willy J.M. UNDERBERG

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Keywords: Befunolol, dihydrobefunolol, high performance liquid chromatography, rabbit plasma, bioavailability, pharmacokinetics

JOURNAL FREE ACCESS

1989 Volume 5 Issue 4 Pages 395-397

DOI https://doi.org/10.2116/analsci.5.395

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Abstract

A simple and rapid high performance liquid chromatographic method has been developed for the determination of befunolol (BF) and its major metabolite, dihydrobefunolol (DBF), in rabbit plasma. The drug and its metabolite were simultaneously detected with a dual-wavelength UV detector to optimize sensitivity. The detection limits were 1ng/ml for BF and 5ng/ml for DBF. This method was shown to be practically applicable to pharmacokinetic studies of BF.

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