Isocratic Separation of Adenosine 5′-Triphosphate and Its Metabolites by Reversed-Phase High Performance Liquid Chromatography: End-Capped versus Uncapped Packings

Takashi NISHIKAWA; Satomi SUZUKI; Hideki OHTANI; Masatoshi SHIRAI; Susumu NOMIYAMA; Hiroaki KUBO

doi:10.2116/analsci.7.241

Isocratic Separation of Adenosine 5′-Triphosphate and Its Metabolites by Reversed-Phase High Performance Liquid Chromatography: End-Capped versus Uncapped Packings

Takashi NISHIKAWA, Satomi SUZUKI, Hideki OHTANI, Masatoshi SHIRAI, Susumu NOMIYAMA, Hiroaki KUBO

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Keywords: High performance liquid chromatography, end-capped packing, uncapped packing, ion-pair chromatography, ATP metabolite, nucleotide, nucleoside

JOURNAL FREE ACCESS

1991 Volume 7 Issue 2 Pages 241-246

DOI https://doi.org/10.2116/analsci.7.241

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Abstract

ATP and its metabolites, such as ADP, AMP, adenosine, inosine and hypoxanthine, were separated by reversed-phase high performance liquid chromatography with isocratic elution on uncapped and capped silica-based columns, and detected by measuring the absorbance at 254nm. An investigation of the effects of an organic solvent as well as the ionic strength, ion-pairing reagents and pH of the mobile phase allowed us to establish chromatographic conditions which are suitable for the determination of these compounds in biological specimens.

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