1991 Volume 7 Issue Supple Pages 1689-1692
Diaphorase (DI)-catalyzed electrolytic oxidation of NADH to NAD+ is studied in the presence of flavins and quinones as electron transfer mediators. The behavior of the catalytic current at the mediated DI-electrode is well explained by theoretical equations based on polarographic catalytic current theory using the reaction layer approximation. The bimolecular reaction rate constants between DI and the mediators are determined by the electrochemical method.