COOKSON-TYPE REAGENTS APPLICATION TO THE DETERMINATION OF 7-DEHYDROCHOLESTEROL IN HUMAN SKIN SURFACE

TOMOYUKI OE; TATSUHITO MIZUGUCHI; KAZUTAKE SHIMADA

doi:10.2116/analsci.7.Supple_171

COOKSON-TYPE REAGENTS

APPLICATION TO THE DETERMINATION OF 7-DEHYDROCHOLESTEROL IN HUMAN SKIN SURFACE

TOMOYUKI OE, TATSUHITO MIZUGUCHI, KAZUTAKE SHIMADA

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Keywords: Cookson-type reagent, 4-(1-anthryl)-1,2,4-triazoline-3,5-dione, high performance liquid chromatography, 7-dehydrocholesterol, human skin surface

JOURNAL FREE ACCESS

1991 Volume 7 Issue Supple Pages 171-172

DOI https://doi.org/10.2116/analsci.7.Supple_171

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Abstract

One of the Cookson-type reagents, 4-(1-anthryl)-1, 2, 4-triazoline-3, 5-dione, has been applied to the determination of 7-dehydrocholesterol in human skin surface. The extract from human skin surface was derivatized with the reagent and the obtained adduct was selectively detected using a UV detector.

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