Abstract
A sensitive and selective method for the simultaneous determination of ribonucleosides (adenosine, cytidine, guanosine, inosine, uridine and xanthosine) and 2′-deoxyribonucleosides (2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine and thymidine) of biological importance (ten species in all) is described. High-performance liquid chromatography with precolumn fluorescence derivatization using 2-(5-chlorocarbonyl-2-oxazolyl)-5, 6-methylenedioxybenzofuran is employed. The reagent reacts selectively with 5′-hydroxyl group of the sugar moieties in the nucleosides to produce the corresponding fluorescent ester derivatives. The resulting ten esters are separated isocratically by chromatography on a reversed phase column, TSKgel ODS-80TM, and detected fluorometrically. The detection limits (signal-to-noise ratio=3) for the nucleosides are 2-12pmol on column.
