Abstract
The influence of chromatographic conditions on the binding capacities (Wcapa) of hydroxyapatite (HAP) for globular proteins was studied. The amount of proteins adsorbed on HAP depended on the loading method. The Wcapa values were affected by the concentration of sodium phosphate buffer, which was interpreted on the basis of a change in the area of the adsorbing surface of the HAP crystal. The Wcapa values were also affected by pH of the buffer, which would be related to both size and charge effects of a protein molecule. The adsorption behavior in the presence of two kinds of proteins was affected by the combination, loading procedure and load ratio of the two tested proteins.
