Abstract
The separation of organophosphorus pesticides by supercritical fluid chromatography was investigated using four packed columns and five fused silica capillary columns. Fourteen pesticides used worldwide were selected; the separation of as many species as possible with one injection was attempted by optimizing the column temperature and the pressure program. Among the packed columns used, an ODS column resulted in a favorable separation, enabling the simultaneous identification of 12 species. However, pesticides having strong hydrogen-bonding functional groups were either not eluted or eluted later. By using capillary columns the chromatographic peaks of these pesticides could be obtained, and various separation modes were possible with liquid stationary phases having different polarities. Also presented is information concerning the retention behavior of each organophosphorus pesticide.
