Chemiluminescent Assays for β-D-Galactosidase and Alkaline Phosphatase Using Novel Luminol Derivatives as Substrates

Manabu NAKAZONO; Hitoshi NOHTA; Yosuke OHKURA; Kazumi SASAMOTO

doi:10.2116/analsci.8.779

Manabu NAKAZONO, Hitoshi NOHTA, Yosuke OHKURA, Kazumi SASAMOTO

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Keywords: Chemiluminescent assay, β-D-galactosidase, alkaline phosphatase, o-aminophthalylhydrazide-β-D-galactoside, o-aminophthalylhydrazide-o-phosphate, chemiluminogenic substrate, luminol

JOURNAL FREE ACCESS

1992 Volume 8 Issue 6 Pages 779-783

DOI https://doi.org/10.2116/analsci.8.779

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Abstract

Chemiluminescent assays for β-D-galactosidase and alkaline phosphatase are described which use novel chemiluminogenic substrates, o-aminophthalylhydrazide-β-D-galactoside and o-aminophthalylhydrazide-O-phosphate, respectively. Each substrate is enzymatically hydrolyzed under the optimum conditions to release luminol, which luminesces in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The detection limits (S/N=3) of the enzyme activities (μU per tube) were 50 for β-D-galactosidase and 100 for alkaline phosphatase.

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