Abstract
A flow-injection analysis system for the determination of ethanol was developed incorporating an oxygen electrode and an enzyme reactor packed with Sepharose 4B on which alcohol dehydrogenase (ADH) and NADH oxidase (NOD) from Bacillus licheniformiswere co-immobilized. Although the response of the system depended on the concentration of FAD added into a carrier solution, it was linearly related to the ethanol concentration over the range 0.25-1.0mM, even in the absence of FAD, by controlling the immobilized amount of ADH and NOD. The linear relationship between the ethanol concentration and the response in the presence of 60μM FAD was observed over the range 0.1-1.5mM. When the system was applied to the determination of ethanol in various kinds of alcoholic beverages, the results obtained under both conditions were comparable to those obtained by the F-kit method.
