STRUCTURE DETERMINATIONS OF ENZYMATICALLY PHOSPHORYLATED PRODUCTS OF AMINOGLYCOSIDIC ANTIBIOTICS BY PROTON MAGNETIC RESONANCE

Abstract

The enzymatically inactivated products of kanamycin, paromamine and dihydrostreptomycin have been investigated by proton magnetic resonance at 100MHz in deuterium oxide solution. The low-field shift of resonance position and the coupling constant of P-O-C-H support the previously proposed structures viz kanamycin-3r-phosphate, paromamine-3'-phosphate and dihydrostreptomycin-3''-phosphate, respectively.