The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
MECHANISM OF ACTION OF SHOWDOMYCIN. V
REDUCED ABILITY OF SHOWDOMYCIN-RESISTANT MUTANTS OF ESCHERICHIA COLI K-12 TO TAKE UP SHOWDOMYCIN AND NUCLEOSIDES
YOSHIHIDE KOMATSU
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1971 Volume 24 Issue 12 Pages 876-883

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Abstract

I have isolated a number of mutants from Escherichia coli K-12 which are highly resistant to showdomycin [2-(β-D-ribofuranosyl)maleimide]. These mutants were very sensitive to N-ethylmaleimide, which has a structure similar to the aglycone moiety of showdomycin. These mutants took up little 14C-labeled showdomycin and showed altered ability to take up various labeled nucleosides. However, their ability to take up cytosine arabinoside-3H remained essentially the same as that of the parent strain. The ability of caffeinetreated mutant cells to take up adenosine-3H was very much lower than that of caffeine-treated parent cells. The nature of deoxycytidine-14C uptake by mutant cells was markedly different from that by parent cells.

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