抄録
Multhiomycin inhibited phe-tRNA binding to ribosomes at magnesium concentration greater than 10 mM. Binding of phe-oligonucleotide (RNase T1 digest of phe-tRNA), to ribosomes and the reaction of puromycin with Nacetyl- phe-tRNA in the presence of GTP and S105 supernatant fraction were not affected. The antibiotic inhibited phe-tRNA binding to the 30S ribosomal subunit. Dipeptide synthesis between phe-tRNA and N-acetyl-phe-tRNA which was prebound to ribosomes was strongly inhibited at 10 mM Mg++. The results suggest that multhiomycin inhibits phe-tRNA binding to aminoacyl site (A site) on the ribosomes.
