PREPARATION OF ¹⁴C-LABELED SHOWDOMYCIN

Abstract

When Streptomyces shoivdoensis (N₂-209-56) mycelia were grown in the presence of sodium acetate-2-¹⁴C, the radioactive carbon from acetate-2-¹⁴C was fairly well incorporated into the showdomycin molecule ; with approximately 14 % of the total radioactivity of the added sodium acetate-2-¹⁴C being incorporated during a 10-hour incubation. Although three antibiotic substances were detected in the fermentation broth, the radioactive carbon from acetate-2-¹⁴C was incorporated only into showdomycin. The radioactive showdomycin was extracted with methanol from the lyophilized powder of the fermentation broth, re-extracted with n-butanol, and finally purified by chromatography on a silicic acid column. Over 98.1% of the total radioactivity of this preparation was due to the ¹⁴C-showdomycin and approximately 0.33 μCi of the preparation corresponded to 1μmole of showdomycin. About 2.8 % of the radioactivity of the added sodium acetate-2-¹⁴C was recovered as that of the purified showdomycin-¹⁴C.