The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Volume 24 , Issue 8
Showing 1-12 articles out of 12 articles from the selected issue
  • G. CORONELLI, G. TAMONI, G. BERETTA, G. C. LANCINI
    1971 Volume 24 Issue 8 Pages 491-496
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Two new compounds, pyracrimycins A and B, have been isolated from a Streptomyces species named Streptomyces eridani Coronelli et al. n. sp. The morphological and physiological characteristics of this strain are described in the present paper together with the production and properties of the two metabolites. Pyracrimycin B is biologically inactive whereas pyracrimycin A is active in vitro against both Gram-positive and Gram-negative bacteria.
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  • C. CORONELLI, A. VIGEVANI, B. GAVALLERI, G. G. GALLO
    1971 Volume 24 Issue 8 Pages 497-502
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Pyracrimycin A, a new antibiotic isolated from cultures of Streptomyces eridani n. sp. (ATCC 21619), was shown to be trans 3-(l-pyrrolin-2-yl)acrylamide (I). The structure determination was based on chemico-physical properties of I and of its dihydroderivative II. Structure I was confirmed by synthesis of the tetrahydro derivative III.
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  • PARTIAL STRUCTURE OF LIVIDOMYCIN A
    TAKESHI ODA, TOSHITO MORI, YOSHINORI KYOTANI
    1971 Volume 24 Issue 8 Pages 503-510
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The acid methanolysis of lividomycin A gave an amine, which was provisionally designated as C12-amine*, and methyl lividotriosaminide. C12-Amine was degraded to 2-deoxystreptamine and an amino sugar by acid hydrolysis of the N-acetyl derivative. The chemical structure of the amino sugar was determined to be 2-amino-2, 3-dideoxy-D-glucopyranose or 3-deoxy-D-glucosamine from the NMR spectrum of peracetylated methyl glycoside of the amino sugar. An α-glycosidic linkage between 2-deoxystreptamine and 3-deoxy-Dglucosamine was shown by the NMR spectrum of N-acetyl C12-amine, and N, N'-diacetyl-5, 6-di-O-methyl-2-deoxystreptamine was obtained from permethylated N-acetyl C12-amine. Therefore, C12-amine is 4-O-(2-amino-2, 3-dideoxy-α-D-glucopyranosyl)-1, 3-diamino-1, 2, 3-trideoxy-myo-inositol or 3'-deoxy paromamine.
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  • STRUCTURE OF LIVIDOMYCIN A
    TAKESHI ODA, TOSHITO MORI, YOSHINORI KYOTANI, MASAHITO NAKAYAMA
    1971 Volume 24 Issue 8 Pages 511-518
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The acid hydrolysis of methyl lividotriosaminide obtained from the methanolysis of lividomycin A gave D-mannose, D-ribose and neosamine B. The sequence and the position of linkage of these three compounds were determined. 1-O-Acetyl-2, 3, 4, 6-tetra-O-methyl-α-D-mannose and l, 3-di-O-acetyl-2, 5-di-O-methyl-β-D-ribose were formed by acid hydrolysis of di-N-acetyl-deca-Omethyllividomycin A followed by acetylation and other observations. The attachment to 2-deoxystreptamine of lividotriosamine was determined by the formation of di-N-acetyl-6-O-methyl-2-deoxystreptamine. The stereochemistry of the glycosidic linkage was determined by comparison of the NMR spectra of N-acetylated derivatives of lividomycin A and paromomycin. Thus, the chemical structure of lividomycin A was concluded to be 4-O-(2-amino-2, 3-dideoxy-α-D-glucopyranosyl)- 5 -O- [3-O- {4-O-(α-D-mannopyranosyl)-2, 6-diamino-2, 6-dideoxy-α-L-idopyranosyl} -β-D-ribofuranosyl]-1, 3-diamino-1, 2, 3-trideoxy-myo-inositol or mannosyldeoxyparomomycin.
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  • Toyota Kobayashi
    1971 Volume 24 Issue 8 Pages 519-525
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Rat-ascites-hepatoma AH 130 cells were incubated at 37°C for 1 hour with bleomycin at the concentration of 1-30 μg/ml. Then, DNA polymerase was prepared from the cells. The enzymatic activity was about half of the control value. Thymidine kinase similarly prepared from bleomycin-treated cells, did not show such a marked inhibition in their activities. In vitro addition of bleomycin to the DNA polymerase assay system did not produce any appreciable inhibition. Moreover, the antibiotic stimulated the enzymatic activity when native DNA was used as template.
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  • IV. STRUCTURES OF ANTIBIOTICS SF-837 A2, A3 AND A4
    TAKASHI TSURUOKA, SHIGEHARU INOUYE, TAKASHI SHOMURA, NORIO EZAKI, TARO ...
    1971 Volume 24 Issue 8 Pages 526-536
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The present paper presents evidence as to the structures of antibiotics SF-837 A2, A3 and A4, minor components of an antibiotic mixture produced by Streptomyces mycarofaciens. Mass spectrometry of three new macrolides, antibiotics SF-837 A2, A3 and A4, revealed that each is composed of the elements of mycaminose, an O-acyl mycarose and an O-acyl macrocyclic lactone. Microbiological deacylation was employed to correlate antibiotic SF-837 A2 with the parent SF-837. Manganese dioxide oxidation of antibiotics SF-837 and SF-837 A2 yielded compounds identical with SF-837 A3 and A4, respectively. These transformations define the structure and stereochemistry of the three minor components of the SF-837 antibiotic mixture.
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  • EFFECTS OF MULTHIOMYCIN ON phe-tRNA BINDING TO RIBOSOMES AND ON OTHER STEPS IN PROTEIN SYNTHESIS
    TERUO TANAKA, KENJI SAKAGUCHI, HIROSHI YONEHARA
    1971 Volume 24 Issue 8 Pages 537-542
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Multhiomycin inhibited phe-tRNA binding to ribosomes at magnesium concentration greater than 10 mM. Binding of phe-oligonucleotide (RNase T1 digest of phe-tRNA), to ribosomes and the reaction of puromycin with Nacetyl- phe-tRNA in the presence of GTP and S105 supernatant fraction were not affected. The antibiotic inhibited phe-tRNA binding to the 30S ribosomal subunit. Dipeptide synthesis between phe-tRNA and N-acetyl-phe-tRNA which was prebound to ribosomes was strongly inhibited at 10 mM Mg++. The results suggest that multhiomycin inhibits phe-tRNA binding to aminoacyl site (A site) on the ribosomes.
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  • I. PRODUCTION, ISOLATION AND CHARACTERIZATION
    A.D. ARGOUDELIS, M.E. BERGY, T.R. PYKE
    1971 Volume 24 Issue 8 Pages 543-557
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Zorbamycin and its related antibiotics, zorbonomycin B and zorbonomycin C, are three new antibiotics isolated from fermentations of Streptomyces bikiniensis var. zorbonensis. All three antibiotics are active against a variety of Gram-positive and Gram-negative bacteria and various fungi. Zorbamycin as well as zorbonomycins B and C appear to belong to the phleomycin and bleomycin families of antibiotics and to be most closely related to the phleomycins. The three antibiotics described in this paper show differences from the reported phleomycins and are likely new members of this group.
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  • CURTIS E. MEYER
    1971 Volume 24 Issue 8 Pages 558-560
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    This paper reports the isolation and characterization of tirandamycin, a new acidic antibiotic which has the molecular formula C22H27NO7. This compound was isolated as the crystalline sodium salt and appears to be structurally related to streptolydigin.
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  • R.S. GORDEE, T.F. BUTLER, N. NARASIMHACHARI
    1971 Volume 24 Issue 8 Pages 561-565
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Dermostatin is a polyene antibiotic which possesses a typical spectrum of antimicrobial activity. In vivo, by parenteral or oral administration, dermostatin was less effective than amphotericin B against Candida albicans. The in vivo activity of dermostatin was comparable to amphotericin B against Cryptococcus neoformans and Blastomyces dermatitidis ; amphotericin B was more effective than dermostatin against Histoplasma capsulatum. Further studies are needed to determine if dermostatin could be useful in the treatment of cryptococcosis or blastomycosis in man.
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  • YOSHIHIDE KOMATSU
    1971 Volume 24 Issue 8 Pages 566-571
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    When Streptomyces shoivdoensis (N2-209-56) mycelia were grown in the presence of sodium acetate-2-14C, the radioactive carbon from acetate-2-14C was fairly well incorporated into the showdomycin molecule ; with approximately 14 % of the total radioactivity of the added sodium acetate-2-14C being incorporated during a 10-hour incubation. Although three antibiotic substances were detected in the fermentation broth, the radioactive carbon from acetate-2-14C was incorporated only into showdomycin. The radioactive showdomycin was extracted with methanol from the lyophilized powder of the fermentation broth, re-extracted with n-butanol, and finally purified by chromatography on a silicic acid column. Over 98.1% of the total radioactivity of this preparation was due to the 14C-showdomycin and approximately 0.33 μCi of the preparation corresponded to 1μmole of showdomycin. About 2.8 % of the radioactivity of the added sodium acetate-2-14C was recovered as that of the purified showdomycin-14C.
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  • YOITI TITANI
    1971 Volume 24 Issue 8 Pages 572-573
    Published: 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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